Stem cells have been vigorously investigated as critical component of reconstructive therapies for a variety of heart diseases since they are capable to differentiate into functional cardiomyocytes. These cells called collectively stromal vascular fraction (SVF) demonstrate multilineage potential and extensive self-renewal capacity. It has been found that human adipose tissue contains a population of non-characterized cells, which are capable to undergo adipogenic, osteogenic, chondrogenic or myogenic differentiation in vitro as well as in vivo. Human SVF cells differentiate into cardiomyocyte-like cells in the presence of the certain set of myogenesis promoting cytokines.Īdipose tissue is an active endocrine organ that secretes adipokines along with a number of pro-angiogenic cytokines and growth promoting factors (reviewed in ). Incubation of the human SVF cells in the medium that promotes cardiomyocyte differentiation in vitro resulted in formation of myotubule-like structures accompanied by up-regulation of the myocardium-characteristic genes, such as GATA, MEF2C, MYOD1, but not ANP. Morphological transformation of the cells was monitored using optical light microscope, whereas changes in expression of the genes typical for cardiac phenotype were measured by qRT-PCR. Human SVF cells were induced to differentiate by their incubation in Methocult medium in the presence of SCF, IL-3 and IL-6.
The goal of this study was to check whether human SVF cells may differentiate into cardiomyocyte-like entities. Stromal vascular fraction (SVF) cells, which are derived from adipose tissue, may represent a potential source of the cells which undergo phenotypical differentiation into many lineages both in vitro as well as in vivo. All rights reserved.Progenitor cells have been extensively studied and therapeutically applied in tissue reconstructive therapy. Look for Blanco to perform "The Git Up" during Thursday Night Football halftime tonight.Ĭopyright © 2019, ABC Radio. "Now mind you, I was only five, and I had to take all this in," he laughs. "That just meant being productive, one with the world, being vertical and going to find something to do." "She'd come in in the morning and look us in the eyes and say, 'Git up!'" he recalls. While the song's title doesn't appear in the lyrics, Blanco says "The Git Up" was inspired by his grandmother.
when I got finished writing.I ended up dancing through the whole song. He goes on: "I was practicing the steps as I was writing. I don't know what got me to do the heels and the hands at the same time. "I'd be out here lyin' if I told you where the 'whoo hoo hoo' came from," he laughs. "The world needs something to smile at and to be joyful about."Īs Blanco explains, "The Git Up" dance incorporates traditional country line dancing steps like the "cowboy boogie" with his own flavor. "I just feel like my energy on the song was so great and inviting, that it was easy to follow directions from someone with so much joy and positivity," Blanco tells ABC Radio about "The Git Up" dance. Not bad for a track that Blanco says he created just to make people smile. on MTV, CMT, BET, and on a billboard in New York's Times Square. But now, the official video for Blanco Brown's viral hit "The Git Up" premieres Friday at 6 a.m. Edgar Evan Moore IVYou've followed the instructions.
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